The 2-Minute Rule for sbs88
The 2-Minute Rule for sbs88
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The trimmed and filtered reads from Each and every sequencing operate and library in which independently aligned for the GRCh38 reference assembly from the human genome23 using the BWA-MEM algorithm v0.7.1724 Pursuing the GATK pipeline. Somatic mutations had been 1st called with Mutect2. Somatic variants had been annotated and candidate driver genes of colorectal cancers documented by3 and IntOGen34 as well as pan-cancer driver genes reported32 and81 filtered With all the Variant Impact Predictor v93.
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